Organic Solvent Stable Lipase from Pseudomonas sp. BCNU 171
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چکیده
منابع مشابه
High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5.
An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 degrees C and 9.0, respectively. The purified lipase was stable at 45 degrees C and pH 6-9. It exhibited the highest stability in the presence of various org...
متن کاملIsolation, identification, biochemical properties and potential application of an organic solvent-tolerant lipase from Pseudomonas sp. strain NEB-1
Using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and organic solvents. Among the strains, 15 isolates exhibited extreme stability toward organic solvents and high lipolytic activity. The NEB-1 isolate which was later identified as Pseudomonas sp. strain NEB-1 by biochemical tests and 16S rDNA gene sequence an...
متن کاملisolation, identification, biochemical properties and potential application of an organic solvent-tolerant lipase from pseudomonas sp. strain neb-1
using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and organic solvents. among the strains, 15 isolates exhibited extreme stability toward organic solvents and high lipolytic activity. the neb-1 isolate which was later identified as pseudomonas sp. strain neb-1 by biochemical tests and 16s rdna gene sequ...
متن کاملImmobilization of Organic Solvent-Tolerant Lipase from Pseudomonas mendocina M-37 with Potential Synthetic Activities
Praveen Dahiya, Subhash Chand and Neeraj Dilbaghi* Department of Bioand Nanotechnology, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi-110016, India Present address: Amity Institute of Biotechnology, Amity University, Sector 125, Gautam Buddha Nagar,...
متن کاملAn organic solvent-tolerant alkaline lipase from cold-adapted Pseudomonas mandelii: cloning, expression, and characterization.
A gene encoding a novel organic solvent-tolerant alkaline lipase, lipS (GenBank ID JQ071496), was cloned from cold-adapted Pseudomonas mandelii. Recombinant LipS was expressed in Escherichia coli as a 32-kDa soluble protein and was purified by standard procedures. It maintained more than 80% of its activity under alkaline conditions, pH 8-10.5, with an apparent optimum temperature range of 40-5...
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ژورنال
عنوان ژورنال: Journal of Life Science
سال: 2015
ISSN: 1225-9918
DOI: 10.5352/jls.2015.25.3.345